Bioinnovation Lab

WRCB-HiMedia Bioinnovation Lab

2D–3D Bioassay & 3D Bioprinting Platforms

Empowering next-generation bioassays and automated bioprinting systems. A CSR initiative funded by HiMedia Laboratories, the lab takes work from lab-scale R&D to GLP-compliant, scalable bio-tissue models through two parallel, standardised workflows.

Explore the Workflows

Funded by HiMedia Laboratories

WRCB-HiMedia Bioinnovation Lab workspace at IIT Bombay

Parallel tracks, one platform

Stages from R&D to industry

>92%

Cell viability before screening

384

Up to 384-well plates

Two parallel, standardised workflows engineered to converge on a single scalable, industry-ready screening platform.

Parallel Workflows

Targets found in 2D screens are validated in 3D models. Switch tracks to follow each pipeline from optimisation to industry-ready services.

Platform maturity

Stages 1–3

Lab-scale R&D

Stage 4

Scalable, reproducible, industry-ready

2D Bioassay Platform (In Progress)

High-throughput biomolecule and target screening on monolayer cultures, taken from assay design through automation to GLP-validated quantification.

Lab-scale R&D

Stage 01

2D Assay Development & Optimisation

Screening biomolecules and target binding

Assay sensitivity & specificity tuning

Assay parameters & reproducibility setup

Method standardisation for multi-well formats

Lab-scale R&D

Stage 02

Standardisation & Automation

Automation of 2D bioassay workflows

Robust SOP development and validation

Automated quality control (QC) checks

Lab-scale R&D

Stage 03

Validation & Quantification

Validation of automated bioassay systems

Quantification precision & signal stability

Benchmarking against reference standards

GLP-compliant documentation

Industry-ready

Stage 04 · Next

GLPs, Insulin & New Biomarker Targets

Project-based end-to-end services

Academia & industry partnership pipeline

Scale-up screening infrastructure

2D Bioassay Platform (In Progress)

High-throughput biomolecule and target screening on monolayer cultures, taken from assay design through automation to GLP-validated quantification.

Lab-scale R&D

Stage 01

2D Assay Development & Optimisation

Screening biomolecules and target binding

Assay sensitivity & specificity tuning

Assay parameters & reproducibility setup

Method standardisation for multi-well formats

Lab-scale R&D

Stage 02

Standardisation & Automation

Automation of 2D bioassay workflows

Robust SOP development and validation

Automated quality control (QC) checks

Lab-scale R&D

Stage 03

Validation & Quantification

Validation of automated bioassay systems

Quantification precision & signal stability

Benchmarking against reference standards

GLP-compliant documentation

Industry-ready

Stage 04 · Next

GLPs, Insulin & New Biomarker Targets

Project-based end-to-end services

Academia & industry partnership pipeline

Scale-up screening infrastructure

Platform Feature Comparison

How the two tracks differ in models, matrices, throughput and compliance.

Feature / Dimension

Track A

2D Bioassay

Track B

3D Bioprinting

Primary Application

Track A · 2D

High-throughput biomolecule & target screening

Track B · 3D

Physiological disease modeling & organ printing

Biological Model

Track A · 2D

Monolayer cell cultures & primary lysates

Track B · 3D

Spheroids, Organoids & vascularized constructs

Matrix / Scaffold

Track A · 2D

Planar plastic / glass multi-well plates

Track B · 3D

Tunable hydrogels, dECM & bio-inks

Throughput Capacity

Track A · 2D

Ultra-High (96 / 384 well plates)

Track B · 3D

Medium to High (384 well / 96 well / 24 well / 12 well / 6 well automated bioprinting)

Compliance Level

Track A · 2D

GLP-validated quantification & SOPs

Track B · 3D

Preclinical validation & automated medical systems

Core Capability Highlights

Built for reproducibility at every step, from automated handling to living tissue models.

Robotic high-throughput integration

Automated liquid handling across 96, 384 and 1536-well formats for 2D and 3D workflows.

Robotic high-throughput integration

Automated liquid handling across 96, 384 and 1536-well formats for 2D and 3D workflows.

Integrated quality control

Cell viability above 92% is confirmed before every screening run.

3D hydrogel & organoid models

Tunable hydrogels, dECM and bio-inks for spheroids, organoids and vascularised constructs.

Multi-material printing

Stromal, vascular, parenchymal, epithelial, endothelial and iPSC / stem-cell layers in one construct.

Core Lab Infrastructure

Operational facilities for aseptic culture, molecular biology, imaging and sample banking, with advanced manufacturing systems in development.

Operational

General Lab

pH meter, water bath, weighing balances, sonicator, magnetic stirrers, incubators

Reagent preparation & general assays

Operational

Cell & Molecular Biology

Thermal cycler, gel electrophoresis units, blotting system, full microscopy suite

Genomics, imaging & molecular biology

Operational

Culture & Incubation

CO₂ and BOD incubators, biosafety cabinets, autoclave, hot air oven

Aseptic mammalian cell culture & growth

Operational

Cold Chain & Analytics

4 °C, −20 °C and −80 °C storage, liquid N₂ tank, multimode plate reader, SpectraDrop, SPR

Bio-sample banking & molecular detection

In development

Advanced Manufacturing

Automated 3D bioprinter, spheroid & organoid bioreactor, automated liquid handling system

3D tissue models & drug-screening scale-up

Operational

Support Infrastructure

Dedicated computing, UPS backup, dehumidifier, fogger system, UV sterilisation

Analytics, operations & facility safety

Inside the Lab

Biosafety cabinets, incubation, cold storage and imaging, set up for aseptic, reproducible work.

Researcher working in a biosafety cabinet

Analytical balances and bench instruments

BOD incubator, freezers and liquid nitrogen

Researcher preparing samples in a biosafety cabinet

Advanced manufacturing

Signature Capabilities

Automation built with Indian industry partners to take multi-cellular tissue models from bench to scale.

Automated 3D Bioprinter

Multi-head printing of multi-cellular tissue models, developed with Indian industry partners.

In development

Spheroid & Organoid Bioreactor

Low-shear culture for spheroids and organoids, with parameters being optimised for scale-up.

In development

Automated Liquid Handling System

Precise, repeatable dispensing across 2D and 3D bioassay workflows.

Operational

Let’s Build What Comes Next

Screening programmes, bio-ink formulation, GLP assay packages or co-development: tell us what you need and we’ll scope it with you.

Partner with the Lab

Explore

Our Team

Hub & Spoke Model

Funding Support

Infrastructure & Facilities

Updates

Engage

Industry Affiliate Program

Technology Scouting

Contact

office@wiciitb.org

+91 22 2576 5500–5508

309, 3rd Floor,

IITB Monash Research Academy,

IIT Bombay, Powai, Mumbai 400076

309, 3rd Floor,

IITB Monash Research Academy,

IIT Bombay, Powai, Mumbai 400076

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